Heterologous expression of cobalamin dependent class-III enzymes
نویسندگان
چکیده
منابع مشابه
Coenzyme B12 (cobalamin)-dependent enzymes.
The B12 or cobalamin coenzymes are complex macrocycles whose reactivity is associated with a unique cobalt-carbon bond. The two biologically active forms are MeCbl and AdoCbl and their closely related cobamide forms. MeCbl participates as the intermediate carrier of activated methyl groups. During the catalytic cycle the coenzyme shuttles between MeCbl and the highly nucleophilic cob(I)alamin f...
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This article is a report on a symposium held at the March 1997 meeting of the American Society for Pharmacology and Experimental Therapeutics in San Diego. Current developments in the heterologous expression of cytochrome P450, NADPH-cytochrome P450 reductase, glutathione transferase, and UDP-glucuronosyltransferase enzymes are described. Systems include bacteria, insect cells, and transient an...
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Candida utilis has been shown to contain 4.7 pmol of cobalamin per g of wet cell paste. Purification of the cobalamin showed it to be a mixture of methylcobalamin and adenosylcobalamin. Two cobalamin-dependent enzyme systems have been found in the yeast: methylcobalamin-dependent methionine biosynthesis and leucine 2,3-aminomutase. The cobalamin extracted from the yeast is as effective as authe...
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Labyrinthopeptins are class III lantibiotics produced by the actinomycete Actinomadura namibiensis. The most characteristic structural feature is the posttranslationally installed triamino triacid labionin with a quaternary α-carbon. In addition to the unique structure, labyrinthopeptin A2 possess remarkable antiviral and antiallodynic biological activities. To harness the substrate tolerance o...
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This work was presented in part at the meeting ofthe American Society of Human Genetics, San Diego, Calif., October 1977. Dr. Mellman and Dr. Willard are recipients of traineeships from the National Institutes of Health (T01-GM 02299). Dr. Mellman's present address is Laboratory of Cellular Physiology and Immunology, The Rockefeller University, New York 10021. Received for publication 18 May 19...
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ژورنال
عنوان ژورنال: Protein Expression and Purification
سال: 2021
ISSN: 1046-5928
DOI: 10.1016/j.pep.2020.105743